Changes in Metabolite Profiling and Expression Levels of Key Genes Involved in the Terpenoid Biosynthesis Pathway in Garden Sage (Salvia officinalis) under the Effect of Hydrazine Hydrate

Mutagenesis is a highly efficient tool for establishing genetic variation and widely used enhancement in various plants. The key benefit of mutation breeding the prospect enhancing one or several characteristics variety without altering background. In this study, we exposed seeds Salvia officinalis to four concentrations hydrazine hydrate (HZ), i.e., (0%, 0.1%, 0.2%, 0.3%) 6 h. contents terpenoid compounds S. plantlets driven from HZ-treated were determined by GC-MS, which resulted identification total 340 phytochemical compounds; 163 (87.48%), 145 (84.49%), 65 (97.45%), 62 (98.32%), HZ 0.3%), respectively. Furthermore, qRT-PCR system disclose “transcriptional control” twelve TPS genes related terpene biosynthesis, namely, SoGPS, SoMYRS, SoNEOD, SoCINS, SoSABS, SoLINS, SoFPPS, SoHUMS, SoTPS6, SoSQUS, SoGGPS, SoGA2. Altogether, results are likely ensure some positive relationship between chemical mutagen treating seeds, type amount produced terpenes, expression their corresponding genes.